Biochemical analyzer. Sensitive ELISA sandwich immunoassays (Immunodiagnostik AG, Bensheim, Germany) were used for the determination of tumor necrosis factor-a (TNF-a), interleukin6 (IL-6) and C-reactive protein (high sensitive CRP) according to manufacturer's instructions. Erythrocyte sedimentation rate (ESR) was estimated after 1 hr in EDTA anti-coagulated blood.ImmunohistochemistryRepresentativ
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